LOCUS       sequence-59696-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt8A-V5
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(549..560)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgccggcact gcccttaccc agggactggg cactgcctgg ggagcgtgcg cagtaatact
      121 tgctcaccac atgcctacac tggtcacact tgaccgtaca gcaccactgg aatttgcagt
      181 tacagctgct tatgacctca gttttcctct cttccacctg cagcccacac tcagtgcaca
      241 ggcgcccaca gctacgtcgc tcccacctgg atgtgttgtg gctgttctgt aggcactcac
      301 gaccctctgt gccatagatg cccaggctgg aattgcaggt acagtaatct ggtgattcct
      361 ctaaaaagat cagttccgcc tctgcgctag gaaggaaggc ctcagcgggc acccagtggc
      421 cctcggcgct gttcccagct ctcagctgcc gcttatccat ttcaattttc agcgcctggt
      481 catacttggc ctttaggtag tctcccatct cccggaactc agccagctgc agccagcatg
      541 tctgtatgct gcagctccca gagatgccat gacatttgca tgtccttttc atggtggctc
      601 tcactgccag tctgccggcc ctgttgttgt gaagattcat cagggctctg gcatccttcc
      661 ccttctccaa actgtccaca aagagtttgg agatcctttc cccaaattcc acattgtcgc
      721 tgcagcctcc ccagatccag ccatggcctc ctgtttttcc attgtttgac ccatcacagc
      781 cacagttttc gaagtcaccc atgctacagt tcttggtgat gatgtacatg actccagcag
      841 agctgatagc atgtatgaag gaagtctctc tggtagcact tct
//
