LOCUS       sequence-59697-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt8B-V5
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgccgggttt tctcccgggt ttgtgcgcag cgcccccccg cggccgctct gcgcggctac
      121 agaagtactt ggtgacccta cggcggcact gctcgcagcg gactgcgcag caccagtgga
      181 acttgcagtt gcagctggac acggtctcgg cccggcgctc ctccaccgcc agcccgcagt
      241 ccccgcagag ccggcggcag ctgcggcgtt cccagcgacc cagggcccgc ccgcgcctta
      301 ggcactctcg gccttcggtg cccagcagcc ctagcgtttt gttctccagg cagtagtccg
      361 gggagtcctc caggtgcacc agctcccggg tagagatgga gcgaaaggtg tcggcgatgg
      421 cgccgcggcc ggccgcgctg ttgccagcac cctgcagcag gtccaccttg agtgctgcgt
      481 ggtacttctc cttcaggtgc gcgcccacct cgcggaactc gggcagctgc agccaacagg
      541 tctgcgtggt gcagctgcca gacacgccgt ggcacttgca cgtgcgtttc atggtgccct
      601 tcaccgcctt gcggccagcc tcgttgttgt gcaggttcat ggctgcccgt gcatcctgtc
      661 ctgtttccag ggcatcgaca aactgcttgg aaatcgcctc tccgaagccc acattgtcac
      721 tgcagcctcc ccacagccag ccttgtcccc ccagttgccc gttgcgggag tcatcacagc
      781 cacagttatc aaaatctcca aggctgcagt ttctagtcag ggtgtacatg actccagcag
      841 aactgatggc atgcacaaat gctgt
//
