LOCUS       sequence-59699-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt9B-V5
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgccgtgctt gcaggtgtac acaagctcct cctgcacaca ttgctggcac tccacgtagc
      121 agcaccactg cacctggcag tggcaggaga aggccaccag gcggctctgg gtgtcatagc
      181 cccgcccgca gcacaggctg ctgcagctgg cctcccggga gcacacccta cctgctgtgc
      241 caggtgagta cttgctgggc cggcagaagc tgggtgagtc ctccatgtac accaggtccc
      301 cagaccttgg ggccaggcct ttggtgaggc tgccctgcct ggcaggggcc cacagctcta
      361 ggcggcccaa ggcctcattg gtggcactgg acaccttgac agccgagtca tagcgcagtt
      421 tcagcacctg gcccgtctca cggaacgggg agagctgctt ccagcaggtg cgcacggcac
      481 aggagcctga tacgccatgg cacttacacg tggtcctgag gccactcttc acagccttga
      541 tgcccacgtg ggtattgtgg gcgtctgccc gtgcccgcag gtccttgttt cctctcttgg
      601 accccaggaa gttgctcaga aacttggtgc tgtacttgag gttgtcaccg cacacgcccc
      661 actgccaggc ctgccggctc tccagccccg gagagtcatc acaggtgcag cgctccatgc
      721 gcccagcgct gcaggcccgg gccagggtgt gggtgagggc ggcagaggac accgcgtaca
      781 ggaaagctgt ctctttgaag cctctcttga gcaggcccgt cctgccctcc aggctacagt
      841 tccagcgctc atgccggaac tgaaactggc actcaagcag gccgaggtgc gcag
//
