LOCUS       sequence-59702-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt10B-V5
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgcccttaca cacattcacc cactctgtaa ccttgcactc atcacacagc acatagcagc
      121 accagtggaa gcggcaatgg cagcgctcaa ctcgtgtctg ccggagcacg ttgtgcccac
      181 ggccacagca caggctgcca cagccatcca acaggcggct ggtcttgttg caggcccggc
      241 cccttgtccc tggggagccc atagtggggt ctcgctcaca gaagtcagga gacttctcaa
      301 agtagaccag ctctcctgag aggcgacggg gacgcagacg gggctggaag gctccagaat
      361 tgcggttgtg ggtatcaatg aagatggccc ggcccagccg ctccctcaac gccgccccca
      421 ctgcccggaa ctctggggcc gccctccagc atgtcttgaa ctggcagctg cctgatgtgc
      481 catgacactt gcatttccgc ttcaggtttt cagttaccac ctggcgcccc accctgttgt
      541 tgtggattcg cattcgtgcc tggatgtccc ggggagcttc cctggaatcc aagaaatccc
      601 gagagaactt ctctccaaag tccatgtcat ggttacagcc accccattcc catgtgtcct
      661 gggggccagg gctggggctt gagccagggc cagggctggg cagagagtgg gggaaactct
      721 tgcctcggga cagtgcctgc agctgcagca gtttggccct cagccgatcc tgctcaccac
      781 tgcccttcca gccacagcca cagctcacca gcttgcccag gctgcaggcc gtggctactg
      841 cgtgcatgac cccagcagcc ag
//
