LOCUS       sequence-59707-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Zhou W, Dosey TL, Biechele T, Moon RT, Horwitz MS, Ruohola-Baker H
  TITLE     Assessment of hypoxia inducible factor levels in cancer cell lines 
            upon hypoxic induction using a novel reporter construct.
  JOURNAL   PLoS One. 2011;6(11):e27460. doi: 10.1371/journal.pone.0027460. Epub
            2011 Nov 23.
  PUBMED    22132102
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(11):e27460. doi"; pages: " 
            10.1371/journal.pone.0027460. Epub 2011 Nov 23"
COMMENT     Sequence Label: HBR-6U
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(42..60)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(43..60)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(75..94)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(76..94)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(122..141)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          149..721
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 ctaccatggt ggatcctgca aaaagaacaa gtagcttgta ttctatagtg tcacctaaat
       61 caagcttgaa ttcgccctat agtgagtcgt attacaattc tttgccaaaa tgatgagaca
      121 gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca tgaacatggt
      181 tagcagaggg gcccggtttg gactcagagt attttatcct catctcaagc agtgtatatc
      241 attgtaacca taaagagaaa ggcaggatga tgaccaggat gtagttgttt ctaccaataa
      301 gaatatttcc acgccagcca gaatttatat gcagaaatat tctaccttat catttaatta
      361 taacaattgt tctctaaaac tgtgctgaag tacaatataa tataccctga ttgccttgaa
      421 aaaaaagtga ttagagaaag tacttacaat ctgacaaata aacaaaagtg aatttaaaaa
      481 ttcgttacaa atgcaagcta aagtttaacg aaaaagttac agaaaatgaa aagaaaataa
      541 gaggagacaa tggttgtcaa cagagtagaa agtgaaagaa acaaaattat catgagggtc
      601 catggtgata caagggacat cttcccattc taaacaacac cctgaaaact ttgccccctc
      661 catataacat gaattttaca atagcgaaaa agaaagaaca atcaagggtc cccaaactca
      721 ccctgaagtt ctcaggatcg atccggagct ttttgcaaaa gcctagtttt taccaacagt
      781 accggaatgc caagctggaa ttcgag
//
