LOCUS       sequence-59709-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  TITLE     Albelda lab PECAM plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNAI Neo mouse PECAM FL
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     123..139
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     123..139
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattctgca
      121 gatctagaac tagtggatcc cccgggctgc aggaattccg gcatggccgg aagacagaac
      181 tagctgagtg cttcagtctg cagaagtctt tcaggattca gctgaggtgg gcctcagtcg
      241 gcagacaaga tgctcctggc tctgggactc acgctggtgc tctatgcaag cctccaggct
      301 gaggaaaact ccttcaccat caacagcatc catatggaaa gcctgccatc atgggaggtg
      361 atgaatgggc agcaactgac cctggagtgc cttgtggaca tcagcaccac ctcgaaaagc
      421 aggtctcagc accgggtgct gttctataag gacgatgcga tggtgtataa cgtcacctcc
      481 agggagcaca ccgagagcta cgtcattcct caggctcggg tcttccactc cgggaagtac
      541 aaatgcacag tgatgctgaa caacaaggaa aaaaccacga ttgagtacga ggtgaaggtg
      601 catggcgtat ccaagcccaa ggtgacactg gacaaaaagg aggtgacaga aggcggggtc
      661 gtgacggtca attgttcctt gcaagaagaa aagccaccga tcttttttaa aattgaaaaa
      721 ttagaagtgg ggacaaagtt tgtcaagcga aggatagata agacctccaa cgagaacttt
      781 gtgctcatgg aattccccat tgaggcgcag gaccacgtgt tagtgttt
//
