LOCUS       sequence-59722-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Mergenthaler P, Muselmann C, Sunwoldt J, Isaev NK, Wieloch T, 
            Dirnagl U, Meisel A, Ruscher K
  TITLE     A functional role of the cyclin-dependent kinase inhibitor 1 
            (p21(WAF1/CIP1)) for neuronal preconditioning.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Mar;33(3):351-5. doi: 
            10.1038/jcbfm.2012.213. Epub 2013 Jan 9.
  PUBMED    23299246
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2012.213"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-03"; volume: "33"; issue: "3"; pages: "351-5"
COMMENT     Sequence Label: pCAG-p21_T145A
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..579
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQASMTDFYHSKRRLIFSKRK
                     P"
     primer_bind     complement(629..648)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accttcttct ctttcctctc ctgacaggtt ggtgtacagt agcttccacc atggactcag
       61 atctcgagct caagcttcga attcatgtca gaaccggctg gggatgtccg tcagaaccca
      121 tgcggcagca aggcctgccg ccgcctcttc ggcccagtgg acagcgagca gctgagccgc
      181 gactgtgatg cgctaatggc gggctgcatc caggaggccc gtgagcgatg gaacttcgac
      241 tttgtcaccg agacaccact ggagggtgac ttcgcctggg agcgtgtgcg gggccttggc
      301 ctgcccaagc tctaccttcc cacggggccc cggcgaggcc gggatgagtt gggaggaggc
      361 aggcggcctg gcacctcacc tgctctgctg caggggacag cagaggaaga ccatgtggac
      421 ctgtcactgt cttgtaccct tgtgcctcgc tcaggggagc aggctgaagg gtccccaggt
      481 ggacctggag actctcaggg tcgaaaacgg cggcaggcca gcatgacaga tttctaccac
      541 tccaaacgcc ggctgatctt ctccaagagg aagccctaag gatccaccgg tcgccagcgg
      601 ccgctagctc gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      661 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg tgaaatttgt
      721 gatgctattg cttt
//
