LOCUS       sequence-59725-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Ragusa MJ, Stanley RE, Hurley JH
  TITLE     Architecture of the Atg17 complex as a scaffold for autophagosome 
            biogenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. 
            Epub 2012 Dec 6.
  PUBMED    23219485
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.11.028"; journalName: "Cell"; date: 
            "2012-12-21- 21"; volume: "151"; issue: "7"; pages: "1501-12"
COMMENT     Sequence Label: Atg17-Atg31-Atg29-pST39 (Auto 16)
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             64..86
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gggccctgta caagatcctg taaaacgacg gccagtgaat tccccgaatt ccccgggaat
       61 aattttgttt aactttaaga aggagatata catatgaatg ttacagttac tgtttatgat
      121 aaaaatgtca agtacagatt agaagagaat atcaaaaata acaagggtcc cagcaacgat
      181 gatcaaccag catacaataa tgaatcaaag agcacggacg gaagtgatta tgcaatgttt
      241 cccactaaca ttaagtacat atttgaggac aacaatgatg aactagtaga ttcaagtgat
      301 gctgctttga cagcaggaat agataaggtc ggagatgaac ttgaaaatgt cataattgtc
      361 caattggatg aatcgggatc tcttgaggat atcacactaa tcagcgacca atatgagctt
      421 ttgtcacata ggacaaatag tctcagcttg gaggaaaacc aaatgagaac attatcatca
      481 catggtgatg ataaatctaa tgatgaagag gaagaactta gcgttgacag tgatagattc
      541 agagtggact cggacattga gctcgatgta atttcacaat tttgcgatct gtctcctttt
      601 cttcgcgatt tgagtttgaa tgacttaatt aaactctacg tcacacaaaa tgaacaatta
      661 caaatgctct ccaattccgt atga
//
