LOCUS       sequence-59726-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Ragusa MJ, Stanley RE, Hurley JH
  TITLE     Architecture of the Atg17 complex as a scaffold for autophagosome 
            biogenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. 
            Epub 2012 Dec 6.
  PUBMED    23219485
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.11.028"; journalName: "Cell"; date: 
            "2012-12-21- 21"; volume: "151"; issue: "7"; pages: "1501-12"
COMMENT     Sequence Label: Atg17-Atg31-Atg29-pST39 (Auto 16)
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgt cgtactacca tcaccatcac
       61 catcacgatt acgatatccc aacgaccgaa aacctgtatt ttcagggcgc catgattatg
      121 aatagtacaa acacagttgt atatatcaaa gttaagggta ggagaccaca gggattcttg
      181 gatcctccca aatttgagtg gaatggaaca aaggagcgac agctttggac aatggtatca
      241 aatttgaatt attcacaaga tcaaatagat tggcagaatt tatcaaaaat atttgaaacg
      301 cctgaatttt ttcttaaaaa gagaacatat aaattatttg ctgaacactt agagctttta
      361 caactacaat tggagaaaaa aagagatctt gagaagtatt caaatgatca agtgaatgaa
      421 gggatgtctg atttaataca taaatatacc cctactttac aaaatgataa tctattaaat
      481 gtatccgcaa gcccactgac cacggaaaga caggattctg aagaagttga gacagaagta
      541 acaaatgagg cgttacaaca tttgcaaact tccaaaattt tgaacattca caagaaaaca
      601 tcggatagtg agaataagcc taatgacaaa ttggataagg atggtattaa taaagaaatg
      661 gagtgcggta gttcagatga cgatttatct tcgagcttaa gtgttagtaa atctgcgttg
      721 gaagaagcgc taatggacag attgcaattc tga
//
