LOCUS       sequence-59728-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Mergenthaler P, Muselmann C, Sunwoldt J, Isaev NK, Wieloch T, 
            Dirnagl U, Meisel A, Ruscher K
  TITLE     A functional role of the cyclin-dependent kinase inhibitor 1 
            (p21(WAF1/CIP1)) for neuronal preconditioning.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Mar;33(3):351-5. doi: 
            10.1038/jcbfm.2012.213. Epub 2013 Jan 9.
  PUBMED    23299246
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2012.213"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-03"; volume: "33"; issue: "3"; pages: "351-5"
COMMENT     Sequence Label: pCAG-p21_S146A
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             84..578
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTAMTDFYHSKRRLIFSKRK
                     P"
     primer_bind     complement(628..647)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccttcttctc tttcctctcc tgacaggttg gtgtacagta gcttccacca tggactcaga
       61 tctcgagctc aagcttcgaa ttcatgtcag aaccggctgg ggatgtccgt cagaacccat
      121 gcggcagcaa ggcctgccgc cgcctcttcg gcccagtgga cagcgagcag ctgagccgcg
      181 actgtgatgc gctaatggcg ggctgcatcc aggaggcccg tgagcgatgg aacttcgact
      241 ttgtcaccga gacaccactg gagggtgact tcgcctggga gcgtgtgcgg ggccttggcc
      301 tgcccaagct ctaccttccc acggggcccc ggcgaggccg ggatgagttg ggaggaggca
      361 ggcggcctgg cacctcacct gctctgctgc aggggacagc agaggaagac catgtggacc
      421 tgtcactgtc ttgtaccctt gtgcctcgct caggggagca ggctgaaggg tccccaggtg
      481 gacctggaga ctctcagggt cgaaaacggc ggcagaccgc catgacagat ttctaccact
      541 ccaaacgccg gctgatcttc tccaagagga agccctaagg atccaccggt cgccagcggc
      601 cgctagctcg acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      661 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt gaaatttgtg
      721 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      781 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
      841 acctctacaa atgtggtaga tccatttaaa tgttaattaa ctagccatga ccaaaatccc
      901 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
      961 ttga
//
