LOCUS       sequence-59750-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Moroz OV, Moroz YS, Wu Y, Olsen AB, Cheng H, Mack KL, McLaughlin JM,
            Raymond EA, Zhezherya K, Roder H, Korendovych IV
  TITLE     A Single Mutation in a Regulatory Protein Produces Evolvable 
            Allosterically Regulated Catalyst of Nonnatural Reaction.
  JOURNAL   Angew Chem Int Ed Engl. 2013 Apr 29. doi: 10.1002/anie.201302339.
  PUBMED    23630096
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2013 Apr 29. doi: 10.1002/anie.201302339."
COMMENT     Sequence Label: pEXP5-NT-7th R
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(477..497)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(510..527)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             546..568
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(599..618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(600..618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatcacc ctttcacttc gctgtcatac gttgtacaaa ctcttcatag
       61 tttacttgac catcaccatc aatgtctgtt tccctaatca gttcatcaac ttcttcatct
      121 gttagcttct ccccacgatt tgtcatcaca atacgaagtt ctgcagcact aatgtaacca
      181 ttaccatcct tgtcttccac acggaactgt tctctaagtt cttcttcgct atctgtatct
      241 ttcatttttc ttgccatcat tgtcagaaac tctgggaagt caattgtgcc attgccatca
      301 gcgtctactt cattgatcat gtcctgtaat tctgcttctg tggggttctg accaagtgat
      361 ctcatcacag tccccaactc ctttgtagtt atagtaccat caccatcctt gtcaaatagt
      421 gaaaaagctt ctttgaattc tgcaatctgc tcttctgtca gttgatcagc cataagggac
      481 tgaaaataca ggttttcgcc gctgctacca tgatgatgat gatgatgaga accagacatg
      541 gtatatctcc ttcttaaagt taaacaaaat tatttctaga gggaaaccgt tgtggtctcc
      601 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg ggcagcgttg ggtcctggcc
      661 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
      721 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
      781 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtg
//
