LOCUS       sequence-59752-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Mali P, Aach J, Lee JH, Levner D, Nip L, Church GM
  TITLE     Barcoding cells using cell-surface programmable DNA-binding domains.
  JOURNAL   Nat Methods. 2013 Mar 17. doi: 10.1038/nmeth.2407.
  PUBMED    23503053
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Mar 17. doi: 10.1038/nmeth.2407."
COMMENT     Sequence Label: sZF1
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     149..208
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             212..238
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             260..283
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             593..622
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             626..772
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(794..811)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    800..911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc ggcttgggga tatccaccat ggagacagac acactcctgc tatgggtact
      181 gctgctctgg gttccaggtt ccactggtga ctatccatat gatgttccag attatgctgg
      241 ggcccagccg gccagatctg actacaagga tgacgacgat aaatctagac ccggggagcg
      301 ccccttccag tgtcgcattt gcatgcggaa cttttcgcag gactcctccc tgcggcggca
      361 tacccgtact cataccggtg aaaaaccgtt tcagtgtcgg atctgtatgc gaaatttctc
      421 ccggcaggag cacctggtgc ggcatctacg tacgcacacc ggcgagaagc cattccaatg
      481 ccgaatatgc atgcgcaact tcagtgaccc cacctccctg aaccggcacc taaaaaccca
      541 cctgagggga tcccaactag tcaaaagtga actgccgcgg ctgcaggtcg acgaacaaaa
      601 actcatctca gaagaggatc tgaatgctgt gggccaggac acgcaggagg tcatcgtggt
      661 gccacactcc ttgcccttta aggtggtggt gatctcagcc atcctggccc tggtggtgct
      721 caccatcatc tcccttatca tcctcatcat gctttggcag aagaagccac gttaggcggc
      781 cgctcgagat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
      841 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
      901 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
      961 gacagcaagg g
//
