LOCUS       sequence-59758-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Mali P, Aach J, Lee JH, Levner D, Nip L, Church GM
  TITLE     Barcoding cells using cell-surface programmable DNA-binding domains.
  JOURNAL   Nat Methods. 2013 Mar 17. doi: 10.1038/nmeth.2407.
  PUBMED    23503053
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Mar 17. doi: 10.1038/nmeth.2407."
COMMENT     Sequence Label: sZF5
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     153..212
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             216..242
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             264..287
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             597..626
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             630..776
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(798..815)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    804..915
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccactagta acggccgcca
      121 gtgtgctgga attcggcttg gggatatcca ccatggagac agacacactc ctgctatggg
      181 tactgctgct ctgggttcca ggttccactg gtgactatcc atatgatgtt ccagattatg
      241 ctggggccca gccggccaga tctgactaca aggatgacga cgataaatct agacccgggg
      301 agcgcccctt ccagtgtcgc atttgcatgc ggaacttttc gaccaaccag aagctggagg
      361 tgcatacccg tactcatacc ggtgaaaaac cgtttcagtg tcggatctgt atgcgaaatt
      421 tctccgtgcg gcacaacctg cagcggcatc tacgtacgca caccggcgag aagccattcc
      481 aatgccgaat atgcatgcgc aacttcagtc agcaccccaa cctgacccgg cacctaaaaa
      541 cccacctgag gggatcccaa ctagtcaaaa gtgaactgcc gcggctgcag gtcgacgaac
      601 aaaaactcat ctcagaagag gatctgaatg ctgtgggcca ggacacgcag gaggtcatcg
      661 tggtgccaca ctccttgccc tttaaggtgg tggtgatctc agccatcctg gccctggtgg
      721 tgctcaccat catctccctt atcatcctca tcatgctttg gcagaagaag ccacgttagg
      781 cggccgctcg agatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
      841 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
      901 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctg
//
