LOCUS       sequence-59762-e        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Mack KL, Moroz OV, Moroz YS, Olsen AB, McLaughlin JM, Korendovych IV
  TITLE     Reprogramming EF-hands for design of catalytically amplified 
            lanthanide sensors.
  JOURNAL   J Biol Inorg Chem. 2013 Apr;18(4):411-8. doi: 
            10.1007/s00775-013-0985-5. Epub 2013 Feb 19.
  PUBMED    23420322
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00775-013-0985-5"; journalName: "J Biol Inorg Chem"; date:
            "2013-04"; volume: "18"; issue: "4"; pages: "411-8"
COMMENT     Sequence Label: pEXP5-NT-CuSeCat
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(489..509)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(522..539)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             558..580
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(611..630)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(612..630)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcctttcggg ctttgttagc agccggatca ccctttcact tcgctctcat acgttgtaca
       61 aactcttcat agtttacttg accatcacca tcaatgtctg cttccctaat catttcatca
      121 acttcttcat ctgttagctt ctccccacga tttgtcatca caatacgaag ttctgcagca
      181 ctaatgtaac cattaccatc cttgtcttcc atacggaacg cttctctaat ttcttcttcg
      241 ctatctgtat ctttcatttt tcttgccatc attgtcagaa actctgggaa gtcaattgtg
      301 ccattgccat cagcgtctac ttcattgatc atgtcctgta attctgcttc tgtggggttc
      361 tgaccaagtg atctcatcac agtccccaac tcctttgtag ttatagtacc atcaccatcc
      421 ttgtcaaata gtgaaaaagc ttctttgaat tctgcaatct gctcttctgt cagttgatca
      481 gccataaggg actgaaaata caggttttcg ccgctgctac catgatgatg atgatgatga
      541 gaaccagaca tggtatatct ccttcttaaa gttaaacaaa attatttcta gagggaaacc
      601 gttgtggtct ccctatagtg agtcgtatta atttcgcggg atcgagatct cgggcagcgt
      661 tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
      721 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
      781 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
      841 ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat
      901 cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgtagcga
      961 tagcggagtg tatactggct taactatgcg gcatcagagc agat
//
