LOCUS       sequence-59773-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F
  TITLE     Comprehensive interrogation of natural TALE DNA-binding modules and 
            transcriptional repressor domains.
  JOURNAL   Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
  PUBMED    22828628
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-07-24"; volume: "3:968. doi"; pages: " 
            10.1038/ncomms1962"
COMMENT     Sequence Label: pAAV_TALE-TF(VP64)-BB_V3
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..134))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(579..599)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(609..674)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      674..683
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggaaccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaggtctc tgtcaggttg aggggtgctc ccgtgagcgc attgcgccac
      181 gcgtgcaccg cctcgaccgc cgtgactcct ccccgcttcg cgatcttcag caactggccc
      241 gtgtccagct gaaggggagg ccctctcagc tctcccgcga ccgtcaacag ggcctcaagc
      301 gctcgggctc cgctccactg tttccccaca ccgacgatcg cctcatgtgt ggcttccggc
      361 aacgccgcaa tcatgtcctg gtacttgacg gcgaccgtgc caagggctgc agggtgctgc
      421 gacaaggcta cgatgtgggc atgtgtaaac ccatgaccca ccagcgcttc gtgatgctgc
      481 gcgactgtcg acctcacttt gggcttgatc ttttcctgct gctgctgtga atatcccaaa
      541 gttctcaaat ctacggctgc tgggactccg tggataccga ccttccgctt cttctttggg
      601 gcaagcttct tatcgtcatc gtctttgtaa tcaatatcat gatccttgta gtctccgtcg
      661 tggtccttat agtccatggt ggcggatcca ccggtccaac ctgaaaaaaa gtgatttcag
      721 gcaggtgctc caggtaatta aacattaata ccccaccaac caaccatccc ttaaaccctt
      781 acctcttgct cagctaatta cagcccggag gagaagggcc gtcccgcccg ctcacctgtg
      841 ggagtaacgc ggtcagtcag agccggggcg ggcggcgcga ggc
//
