LOCUS       sequence-59777-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Colby DW, Chu Y, Cassady JP, Duennwald M, Zazulak H, Webster JM, 
            Messer A, Lindquist S, Ingram VM, Wittrup KD
  TITLE     Potent inhibition of huntingtin aggregation and cytotoxicity by a 
            disulfide bond-free single-domain intracellular antibody.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17616-21. Epub 2004 
            Dec 14.
  PUBMED    15598740
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-12-21"; volume: "101(51)"; pages: 
            "17616-21. Epub 2004 Dec 14"
COMMENT     Sequence Label: pNES-VL12.3
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             496..513
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(617..634)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    623..847
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaacg ggccctctag aatgggtatc
      121 caggagctgt ccgagcagat ccacaggctg ctgctgtctc agcctgtgct gactcagtca
      181 ccctcggtct ctgcagcccc ccggcaaagg gtcaccatct ccgtgtctgg aagcaactcc
      241 aacatcggaa gtaatactgt aaactggatc cagcagctcc caggaagggc tcccgagctc
      301 ctcatgtacg atgacgatct attggcccca ggggtctctg accgattttc tggctctagg
      361 tctggcacct cagcctccct gaccatcagt gggctccagt ctgaggatga ggctgattat
      421 tacgcagcaa catgggatga cagcctgaat ggttgggtgt tcggcggagg gaccaaggtc
      481 accgtcctat ccggacatca ccatcaccat cactagctcg agcggccgcc actgtgctgg
      541 atatctgcag aattccacca cactggacta gtggatccga gctcggtacc aagcttaagt
      601 ttaaaccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
      661 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
      721 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
      781 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
      841 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
      901 tgtaacggcg cattaa
//
