LOCUS       sequence-59792-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Phong C, Markson JS, Wilhoite CM, Rust MJ
  TITLE     Robust and tunable circadian rhythms from differentially sensitive 
            catalytic domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1124-9. doi: 
            10.1073/pnas.1212113110. Epub 2012 Dec 31.
  PUBMED    23277568
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1212113110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-15- 15"; volume: "110"; issue: "3"; pages: "1124-9"
COMMENT     Sequence Label: KaiC CII cat- (E318Q)
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..98
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(102..110,111..125)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             135..158
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc
       61 atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg
      121 ataaggatcc gggactggaa gttctgttcc aggggcccat gacttccgct gagatgacta
      181 gccctaataa taattctgag caccaagcca tcgctaagat gcgcacgatg attgaaggct
      241 ttgatgatat tagtcatggc ggtcttccaa tcgggcgatc gaccctcgtt agtggtactt
      301 caggaaccgg caagaccctt ttttctattc aatttctcta taacggtatt atcgagtttg
      361 atgagcctgg ggttttcgtt actttcgaag aaaccccgca agatatcatt aaaaacgccc
      421 gtagttttgg ctgggattta gccaagctgg tcgatgaggg caaactattt attcttgatg
      481 cttcacccga tccagaaggt caagaggttg ttggcggctt cgatctctct gctctgattg
      541 agcggattaa ttatgcaatt caaaagtatc gagcgcggcg ggtttcaatt gactcggtca
      601 cgtccgtttt ccagcaatat gatgcctctt ctgtggttcg ccgcgaactc tttcggttgg
      661 tagctcgcct aaaacaaatt ggggcaacta cggtcatgac caccgagcgt atcgaggaat
      721 atggcccgat cgctcgttac ggtgttgagg aatttgtctc cgataacgtc gtgattctcc
      781 gcaacgtttt ggaaggggag cgccgtcgcc gcaccctcga aatcctcaag ctacgtggca
      841 ccagccacat gaaaggggaa tatccgttca cgattacgga tcatggcatc aatatcttcc
      901 cgctcggggc aatgcgcctt acgcagcgat cgtcgaacgt gcgtgtttca tc
//
