LOCUS       sequence-59794-e       11062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11062)
  AUTHORS   Patel M, Giddings AM, Sechelski J, Olsen JC
  TITLE     High efficiency gene transfer to airways of mice using influenza 
            hemagglutinin pseudotyped lentiviral vectors.
  JOURNAL   J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695.
  PUBMED    23319179
REFERENCE   2  (bases 1 to 11062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jgm"; 
            journalName: "J Gene Med"; date: "2013-01"; volume: "15"; issue: 
            "1"; pages: "51-62"
COMMENT     Sequence Label: pEIAV-SIN6.1 CBLacZ-LucWm1A
FEATURES             Location/Qualifiers
     source          1..11062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        146..525
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        526..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2612..5656
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(2614..2636)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    complement(5676..5700)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5726..5791
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             5822..7471
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKALKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5885..5903)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             complement(8161..8172)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      8484..8939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8571..8590)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8781..8802
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9008..9027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9127..9149
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9187..9205)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9273..9377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9378..9446,9447..10238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9596..9615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10409..10997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10898..10917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acagatcttc aatattggcc attagccata ttattcattg gttatatagc ataaatcaat
       61 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
      121 tcatgtccaa tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgggcactc agattctgcg gtctgagtcc cttctctgct
      781 gggctgaaaa ggcctttgta ataaatataa ttctctactc agtccctgtc tctagtttgt
      841 ctgttcgaga tcctacagtt ggcgcccgaa cagggacctg agaggggcgc agaccctacc
      901 tgttgaacct ggctgatcgt aggatccccg ggacagcaga ggagaactta cagaagtctt
      961 ctggaggtgt tcctggccag aacacaggag gacaggtaag tagggagacc ctttgacatg
     1021 gagcaaggcg ctcaagaagt tagagaaggt gacggtacaa gggtctcaga aattaactac
     1081 tggtaactgt aattgggcgc taagtctagt agacttattt catgatacca actttgtaaa
     1141 agaaaaggac tggcagctga gggatgtcat tccattgctg gaagatgtaa ctcagacgct
     1201 ggaattcggg tattgtagaa agggcaaata ggaccttgaa agagaagatt caaagtcata
     1261 gagacaacac tcaaacactg gaggcagctt tacaacttgc tctcattact tgtaacaaag
     1321 ggagggaaag tatgggagga cagacaccat gggaagtatt tatcactaat caagcacaag
     1381 taatacatga gaaactttta ctacagcaag cacaatcctc caaaaaattt tgtttttaca
     1441 aaatccctgg tgaacatgat tggaagggac ctactagggt gctgtggaag ggtgatggtg
     1501 cagtagtagt taatgatgaa ggaaagggaa taattgctgt accattaacc aggactaagt
     1561 tactaataaa gccaaattga gtattgttgc atgcatttga tatatgggat tatttggtaa
     1621 aggggtaaca tggtcagcat cgcattctat ggggggatcc cagggggaat ctcaacccct
     1681 attacccaac agtcagaaaa atctaagtgt gaggagaaca caatgtttca accttattgt
     1741 tataataatg acagtaagaa cagcatggca gaatcgaagg aagcaagaga ccaagaaatg
     1801 aacctgaaag aagaatctaa agaagaaaaa agaagaaatg actggtggaa aaaagctgca
     1861 gatagctagc ggtacctctg gtcgttacat aacttacggt aaatggcccg cctggctgac
     1921 cgcccaacga ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1981 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2041 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2101 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2161 ctcgaggcca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
     2221 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc
     2281 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
     2341 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
     2401 cggccctata aaaagcgaag cgcgcggcgg gcgggagcgg gatcagccac cgcggtggcg
     2461 gcctagagtc gagttgggga tcgaaagagc ctgctaaagc aaaaaagaag tcaccatgtc
     2521 gtttactttg accaacaaga acgtgatttt cgttgccggt ctgggaggca ttggtctgga
     2581 caccagcaag gagctgctca agcgcgatcc cgtcgtttta caacgtcgtg actgggaaaa
     2641 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2701 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2761 gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct
     2821 tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc
     2881 catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa
     2941 tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca
     3001 gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg
     3061 ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg
     3121 cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg agtgacggca gttatctgga
     3181 agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc
     3241 gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc
     3301 tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt
     3361 ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat
     3421 tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc
     3481 gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc
     3541 cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga
     3601 aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga
     3661 gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct
     3721 gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg
     3781 gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca
     3841 cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga
     3901 acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct
     3961 ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt
     4021 cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat
     4081 tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg
     4141 gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata
     4201 cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca
     4261 gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata
     4321 tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga
     4381 tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac
     4441 ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt
     4501 gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct
     4561 ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca
     4621 gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt
     4681 acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca
     4741 gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc
     4801 gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt
     4861 taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac
     4921 gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc
     4981 gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc
     5041 cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac
     5101 cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat
     5161 tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca
     5221 tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct
     5281 cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg
     5341 ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg
     5401 ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa
     5461 catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc
     5521 ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc
     5581 ctggagcccg tcagtatcgg cggaattaca gctgagcgcc ggtcgctacc attaccagtt
     5641 ggtctggtgt caaaaaacta agggtgggcg cgccgaccca gctttcttgt acaaagtggt
     5701 tgatatccag cacagtggcg gccgcgtgaa acagactttg aattttgacc ttcttaagct
     5761 tgcgggagac gtcgagtcca atccgggccc gcggtccggt actgttggta aagccacctt
     5821 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
     5881 cgctggagag caactgcata aggctttgaa gagatacgcc ctggttcctg gaacaattgc
     5941 ttttacagat gcacatatcg aggtagacat cacttacgct gagtacttcg aaatgtccgt
     6001 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
     6061 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
     6121 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
     6181 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
     6241 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
     6301 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
     6361 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
     6421 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
     6481 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
     6541 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
     6601 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa
     6661 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat
     6721 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa
     6781 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg
     6841 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     6901 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     6961 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta
     7021 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     7081 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct
     7141 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca
     7201 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc
     7261 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     7321 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     7381 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     7441 ggccaagaag ggcggaaaga tcgccgtgta attctagagt cgacccgggc ggccgccgag
     7501 catcttaccg ccatttattc ccatatttgt tctgtttttc ttgatttggg tatacatttg
     7561 attgtcaata aaacaaaatg gtggggcaat catctacatt tcatgggata tgtgattact
     7621 agttcaggtg tattgccaca agacaaactt gttaagaaaa tttcccgtta tttgcactct
     7681 gttcctgtta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7741 tatgttgctc cttttacgct ttgtggatac gctgctttaa tgcctttgta tcttgctatt
     7801 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7861 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7921 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7981 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     8041 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcct
     8101 aggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8161 tcggccctca atccagcgga ccttccttcc cgcggatgca tctccgatcg attagaaaaa
     8221 caagggggga actgtggggt ttttatgagg ggttttatac aattgggcac tcagattctg
     8281 cggtctgagt cccttctctg ctgggctgaa aaggcctttg taataaatat aattctctac
     8341 tcagtccctg tctctagttt gtctgttcga gatcctacag agctcatgcc ttggttaatt
     8401 aaggagatcc gggctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8461 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8521 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8581 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     8641 gctcccttta gggttccgat ttagagcttt acggcacctc gaccgcaaaa aacttgattt
     8701 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8761 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8821 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     8881 tgagctgatt taacaaatat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc
     8941 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     9001 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     9061 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     9121 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     9181 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     9241 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9301 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9361 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9421 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9481 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9541 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9601 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9661 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9721 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9781 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9841 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9901 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9961 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10021 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10081 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10141 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10201 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10261 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10321 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10381 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10441 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10501 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    10561 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10621 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10681 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10741 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10801 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10861 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10921 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10981 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11041 ggccttttgc tcacatggct cg
//
