LOCUS       sequence-59795-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Phong C, Markson JS, Wilhoite CM, Rust MJ
  TITLE     Robust and tunable circadian rhythms from differentially sensitive 
            catalytic domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1124-9. doi: 
            10.1073/pnas.1212113110. Epub 2012 Dec 31.
  PUBMED    23277568
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1212113110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-15- 15"; volume: "110"; issue: "3"; pages: "1124-9"
COMMENT     Sequence Label: KaiC CI cat-/EE (E77Q; E78Q; S431E; T432E)
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..100
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(104..112,113..127)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             137..160
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgcgg ggttctcatc atcatcatca
       61 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga
      121 cgataaggat ccgggactgg aagttctgtt ccaggggccc atgacttccg ctgagatgac
      181 tagccctaat aataattctg agcaccaagc catcgctaag atgcgcacga tgattgaagg
      241 ctttgatgat attagtcatg gcggtcttcc aatcgggcga tcgaccctcg ttagtggtac
      301 ttcaggaacc ggcaagaccc ttttttctat tcaatttctc tataacggta ttatcgagtt
      361 tgatgagcct ggggttttcg ttactttcca gcagaccccg caagatatca ttaaaaacgc
      421 ccgtagtttt ggctgggatt tagccaagct ggtcgatgag ggcaaactat ttattcttga
      481 tgcttcaccc gatccagaag gtcaagaggt tgttggcggc ttcgatctct ctgctctgat
      541 tgagcggatt aattatgcaa ttcaaaagta tcgagcgcgg cgggtttcaa ttgactcggt
      601 cacgtccgtt ttccagcaat atgatgcctc ttctgtggtt cgccgcgaac tctttcggtt
      661 ggtagctcgc ctaaaacaaa ttggggcaac tacggtcatg accaccgagc gtatcgagga
      721 atatggcccg atcgctcgtt acggtgttga ggaatttgtc tccgataacg tcgtgattct
      781 ccgcaacgtt ttggaagggg agcgccgtcg ccgcaccctc gaaatcctca agctacgtgg
      841 caccagccac atgaaagggg aatatccgtt cacgattacg gatcatggca tcaatatctt
      901 cccgctcggg gcaatgcgcc ttacgcagcg atcgtcgaac gtgcgtgttt catctggtgt
      961 cgtccg
//
