LOCUS       sequence-59798-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Phong C, Markson JS, Wilhoite CM, Rust MJ
  TITLE     Robust and tunable circadian rhythms from differentially sensitive 
            catalytic domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1124-9. doi: 
            10.1073/pnas.1212113110. Epub 2012 Dec 31.
  PUBMED    23277568
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1212113110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-15- 15"; volume: "110"; issue: "3"; pages: "1124-9"
COMMENT     Sequence Label: KaiC CI cat- (E77Q;E78Q)
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..102
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(106..114,115..129)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             139..162
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc ggggttctca tcatcatcat
       61 catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat
      121 gacgataagg atccgggact ggaagttctg ttccaggggc ccatgacttc cgctgagatg
      181 actagcccta ataataattc tgagcaccaa gccatcgcta agatgcgcac gatgattgaa
      241 ggctttgatg atattagtca tggcggtctt ccaatcgggc gatcgaccct cgttagtggt
      301 acttcaggaa ccggcaagac ccttttttct attcaatttc tctataacgg tattatcgag
      361 tttgatgagc ctggggtttt cgttactttc cagcaaaccc cgcaagatat cattaaaaac
      421 gcccgtagtt ttggctggga tttagccaag ctggtcgatg agggcaaact atttattctt
      481 gatgcttcac ccgatccaga aggtcaagag gttgttggcg gcttcgatct ctctgctctg
      541 attgagcgga ttaattatgc aattcaaaag tatcgagcgc ggcgggtttc aattgactcg
      601 gtcacgtccg ttttccagca atatgatgcc tcttctgtgg ttcgccgcga actctttcgg
      661 ttggtagctc gcctaaaaca aattggggca actacggtca tgaccaccga gcgtatcgag
      721 gaatatggcc cgatcgctcg ttacggtgtt gaggaatttg tctccgataa cgtcgtgatt
      781 ctccgcaacg ttttggaagg ggagcgccgt cgccgcaccc tcgaaatcct caagctacgt
      841 ggcaccagcc acatgaaagg ggaatatccg ttcacgatta cggatcatgg catcaatatc
      901 ttcccgctcg gggcaatgcg ccttacgcag cgatcgtcga acgtgcgtgt ttcatctggt
      961 gtcgtccga
//
