LOCUS       sequence-59803-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT,
            Yeh JR, Joung JK
  TITLE     Efficient genome editing in zebrafish using a CRISPR-Cas system.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
  PUBMED    23360964
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501."
COMMENT     Sequence Label: MLM3639
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(36..101)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(108..128)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggtgatgg tgatgatgac cggtcatcct gcagccttgt catcgtcatc cttgtaatcg
       61 atgtcatgat ctttataatc accgtcatgg tctttgtagt cgctcgagac tttcctcttc
      121 ttcttgggag aaccgtcacc tcctagctga ctcaaatcaa tgcgtgtttc ataaagacca
      181 gtgatggatt gatggataag agtggcatct aaaacttctt ttgtagacgt atatcgttta
      241 cgatcaattg ttgtatcaaa atatttaaaa gcagcgggag ctccaagatt cgtcaacgta
      301 aataaatgaa taatattttc tgcttgttca cgtattggtt tgtctctatg tttgttatat
      361 gcactaagaa ctttatctaa attggcatct gctaaaataa cacgcttaga aaattcactg
      421 atttgctcaa taatctcatc taaataatgc ttatgctgct ccacaaacaa ttgtttttgt
      481 tcgttatctt ctggactacc cttcaacttt tcataatgac tagctaaata taaaaaattc
      541 acatatttgc ttggcagagc cagctcattt cctttttgta attctccggc actagccagc
      601 atccgtttac gaccgttttc taactcaaaa agactatatt taggtagttt aatgattaag
      661 tcttttttaa cttccttata tcctttagct tctaaaaagt caatcggatt tttttcaaag
      721 gaacttcttt ccataattgt gatccctagt aactctttaa cggattttaa cttcttcgat
      781 ttcccttttt ccaccttagc aaccactagg actgaataag ctaccgttgg actatcaaaa
      841 ccaccatatt tttttggatc ccagtctttt ttacgagcaa taagcttg
//
