LOCUS       sequence-59810-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET19b converted Gateway Destination vector for expressing His 
            tagged fusion proteins in bacteria. See note in comments section 
            below regarding the mutated enterokinase site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZY19
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
       61 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
      121 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      181 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      241 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      301 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
      361 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
      421 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
      481 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
      541 agtggcaggg ggggcgtaaa cgccgcgtgg atccggctta ctaaaagcca gataacagta
      601 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
      661 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
      721 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
      781 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
      841 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgac
//
