LOCUS       sequence-59821-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-CCTA
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(614..630)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(614..630)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(627..649)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    638..654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(662..668,669..686,687..692))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    707..728
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(845..862)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatagggcga attgggccct ctagatgcat gctcgagcgg ccgccagtgt gatggatatc
       61 tgcagaattc gcccttatat agatgccgtc ctagcgcgtc tcctgacccc agagcaggtc
      121 gtggcgatcg caagccacga cggaggaaag caagccttgg aaacagtaca gaggctgttg
      181 cctgtgctgt gccaagcgca cggactcacc ccagagcagg tcgtggcgat cgcaagccac
      241 gacggaggaa agcaagcctt ggaaacagta cagaggctgt tgcctgtgct gtgccaagcg
      301 cacggcctca ccccagagca ggtcgtggcc attgcctcga atggaggggg caaacaggcg
      361 ttggaaaccg tacaacgatt gctgccggtg ctgtgccaag cgcacggatt aaccccagag
      421 caggtcgtgg caatcgcctc caacattggc gggaaacagg cactcgagac tgtccagcgc
      481 ctgcttcccg tgctgtgcca agcgcacggc ttaagagacg tgggcacagg aaagatactt
      541 aagggcgaat tccagcacac tggcggccgt tactagtgga tccgagctcg gtaccaagct
      601 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      661 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      721 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      781 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      841 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      901 actcaaaggc ggtaatacgg ttatcc
//
