LOCUS       sequence-59824-e        4307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Patel M, Giddings AM, Sechelski J, Olsen JC
  TITLE     High efficiency gene transfer to airways of mice using influenza 
            hemagglutinin pseudotyped lentiviral vectors.
  JOURNAL   J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695.
  PUBMED    23319179
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jgm"; 
            journalName: "J Gene Med"; date: "2013-01"; volume: "15"; issue: 
            "1"; pages: "51-62"
COMMENT     Sequence Label: pCI-M2
FEATURES             Location/Qualifiers
     source          1..4307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1419..1540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1430..1449)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1484..1503
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1721..2176
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1808..1827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2018..2039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2245..2264)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2364..2386
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2424..2442)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2510..2614
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2615..2683,2684..3475)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2833..2852)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3646..4234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4135..4154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattcac gcgtggtacc
     1081 tctagaggat ccatgagtct tctaaccgag gtcgaaacgc ctaccagaaa cggatgggag
     1141 tgcagatgca acgattcaag tgaccctctc attattgccg caagtatcat tgggatcttg
     1201 cacttgatat tgtggattct taatcgtctt ttcttcaaat gcatttatcg tcgccttaaa
     1261 tacggtttga aaagagggcc ttctacggaa ggagtgcctg agtctatgag ggaagaatat
     1321 cggcaggaac agcagagtgc tgtggatgtt gacgatggtc attttgtcag catagagctg
     1381 gagtaaggat cccgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg
     1441 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     1501 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     1561 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta
     1621 caaatgtggt aaaatcgata aggatccggg ctggcgtaat agcgaagagg cccgcaccga
     1681 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
     1741 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     1801 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     1861 caagctctaa atcgggggct ccctttaggg ttccgattta gagctttacg gcacctcgac
     1921 cgcaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     1981 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2041 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2101 gcctattggt taaaaaatga gctgatttaa caaatattta acgcgaattt taacaaaata
     2161 ttaacgttta caatttcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     2221 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     2281 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     2341 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     2401 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     2461 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2521 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2581 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2641 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2701 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2761 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2821 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2881 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2941 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3001 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     3061 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     3121 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     3181 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     3241 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3301 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3361 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3421 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3481 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3541 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3601 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3661 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3721 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3781 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3841 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3901 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3961 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4021 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4081 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4141 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4201 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4261 gttcctggcc ttttgctggc cttttgctca catggctcga cagatct
//
