LOCUS       sequence-59826-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: G3BP1A
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             598..615
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(668..686)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      682..729
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(853..872)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggt
      121 gatggagaag cctagtcccc tgctggtcgg gcgggaattt gtgagacagt attacacact
      181 gctgaaccag gccccagaca tgctgcatag attttatgga aagaactctt cttatgtcca
      241 tgggggattg gattcaaatg gaaagccagc agatgcagtc tacggacaga aagaaatcca
      301 caggaaagtg atgtcacaaa acttcaccaa ctgccacacc aagattcgcc atgttgatgc
      361 tcatgccacg ctaaatgatg gtgtggtagt ccaggtgatg gggcttctct ctaacaacaa
      421 ccaggctttg aggagattca tgcaaacgtt tgtccttgct cctgaggggt ctgttgcaaa
      481 taaattctat gttcacaatg atatcttcag ataccaagat gaggtctttg gttgacagta
      541 aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac
      601 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      661 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      721 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
      781 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      841 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      901 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      961 tcgaccccaa
//
