LOCUS       sequence-59829-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NISCHA
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             505..522
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(575..593)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      589..636
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(760..779)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgga
      121 agcgcgcgtc gtgggctcgg agcttgtgga cacttatacg gtttacatca tccaggtcac
      181 tgatggcagc catgagtgga cagtaaagca ccgctacagc gacttccatg acctgcatga
      241 aaagctcgtt gcagagagaa agattgataa aaacctgctt ccgcccaaaa agataattgg
      301 gaaaaactca agaagcttgg tggagaagag ggagaaggat ctggaggtct acctccagaa
      361 gctcctggct gccttccctg gcgtgacccc cagagtactg gcccacttct tgcattttca
      421 cttctatgag ataaatggct aacagtaaag gtggatacgg atccgaattc gagctccgtc
      481 gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      541 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      601 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      661 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      721 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      781 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      841 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      901 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtc
//
