LOCUS       sequence-59830-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIK3R2A
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             757..774
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(827..845)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      841..888
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggg
      121 cctcacactc cccgacttgc ccgagcagtt ctccccacct gatgtggctc cccctcttct
      181 ggtgaagctt gtggaggcca ttgaaaggac agggctggac agcgaatctc actaccgccc
      241 ggagctgccc gcaccgcgta cagactggtc cctgagcgac gtggatcagt gggacacggc
      301 agccctggct gacggcatta agagcttcct gctggcactg cccgcgccgc tcgtgacccc
      361 cgaggcctcg gccgaggcgc gccgggccct gcgggaggcc gcggggcccg tggggccggc
      421 gctggagcca ccgacgctgc cgctgcaccg cgcgctcacg ctgcgcttcc tgctccagca
      481 cctgggccgc gtggcccgcc gcgccccggc cctgggtccc gcggtccggg ccctgggcgc
      541 cacctttggg ccgctgctgc tgcgcgcgcc gccgccgccg tcctcgccgc cgccaggggg
      601 cgctcccgac gggagtgagc ccagccctga cttcccggcg ctgctggtgg agaagctgct
      661 tcaggaacac ttggaagagc aggaggttgc gtgacagtaa aggtggatac ggatccgaat
      721 tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      781 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      841 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      901 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgc
//
