LOCUS       sequence-59831-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ERI3A
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             787..804
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(857..875)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      871..918
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgtc
      121 cttccctccc cagaggtatc actacttttt agtgctggac tttgaggcca cgtgcgacaa
      181 gccacagatt catcctcagg aaatcatcga gttccccatc ctaaagctaa atggccggac
      241 catggagatt gagtctacct ttcacatgta tgtccagcct gtagtccatc cacagcttac
      301 cccattctgt acagagctca ccgggattat tcaagccatg gtggatggtc agccaagcct
      361 gcagcaagtg ctggagaggg tcgatgaatg gatggcgaag gaaggcctct tagatccaaa
      421 cgtcaagtca atttttgtca cctgtggaga ctgggactta aaagtcatgc tcccaggcca
      481 gtgccagtac ttgggcttgc cagtggcgga ttacttcaag cagtggatta atctgaaaaa
      541 ggcttacagc ttcgccatgg gctgctggcc caagaatgga cttctagaca tgaacaaggg
      601 cctcagcctg caacacatag gccggcccca cagcggcatt gacgactgca agaacattgc
      661 caacatcatg aagacactcg cctatcgagg cttcatcttc aagcagacat cgaagccgtt
      721 ctgacagtaa aggtggatac ggatccgaat tcgagctccg tcgacaagct tgcggccgca
      781 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      841 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      901 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      961 cctgtagcgg cgcattaa
//
