LOCUS       sequence-59837-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CPSF7A
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             583..600
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(653..671)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      667..714
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(838..857)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgga
      121 accacctcct cctgttcgcc aggagccatc tcccaagccc aacaacaaga cccctgcaat
      181 tctgtatacc tacagtggcc tgcgtaatag acgagctgcc gtttatgtgg gcagcttctc
      241 ctggtggacc acagaccagc agctgatcca ggttattcgc tctataggag tctatgatgt
      301 ggtggagttg aaatttgcag agaatcgagc aaatggccag tccaaagggt atgctgaggt
      361 ggtggtagcc tctgaaaact ctgtccacaa attgttggaa ctcctaccag ggaaagttct
      421 taatggagaa aaagtggacg tgaggccggc cacccggcag aacctgtcac agtttgaggc
      481 acaggctcgg aaacgtgagt gtgtccgagt cccaagatga cagtaaaggt ggatacggat
      541 ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac
      601 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      661 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      721 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      781 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      841 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      901 atcgggggct ccctttaggg ttccgatt
//
