LOCUS       sequence-59838-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HSPA9A
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             661..678
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(731..749)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      745..792
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgga
      121 tgtcactccc ctgtctctgg gtattgaaac tctaggaggt gtctttacca aacttattaa
      181 taggaatacc actattccaa ccaagaagag ccaggtattc tctactgccg ctgatggtca
      241 aacgcaagtg gaaattaaag tgtgtcaggg tgaaagagag atggctggag acaacaaact
      301 ccttggacag tttactttga ttggaattcc accagcccct cgtggagttc ctcagattga
      361 agttacattt gacattgatg ccaatgggat agtacatgtt tctgctaaag ataaaggcac
      421 aggacgtgag cagcagattg taatccagtc ttctggtgga ttaagcaaag atgatattga
      481 aaatatggtt aaaaatgcag agaaatatgc tgaagaagac cggcgaaaga aggaacgagt
      541 tgaagcagtt aatatggctg aaggaatcat tcacgacaca gaaaccaaga tggaatgaca
      601 gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag
      661 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
      721 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      781 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
      841 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      901 gcgccctagc gcccgctcct tt
//
