LOCUS       sequence-59839-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBF3A
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             655..672
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(725..743)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      739..786
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(910..929)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgtc
      121 agaagccact ccgtgcatca aggccatcag tcccagtgaa ggctggacca cggggggtgc
      181 caccgtcatc ataattggcg acaacttctt tgacgggctg caagttgtat tcggaactat
      241 gttggtgtgg agcgagctga taactcccca tgccatccga gtccagaccc cgccgaggca
      301 cattcctggc gtcgtcgaag tgaccctctc ctacaaatcc aagcagttct gcaaaggtgc
      361 tcctgggcgc tttgtctaca ccgcccttaa tgaaccaacc atagattacg gctttcagag
      421 gttgcagaaa gtgatcccaa gacatccggg tgatcccgaa aggttaccca aggaggtgtt
      481 actgaagcgg gcggcggacc tggtggaagc cttatacgga atgcctcaca acaaccagga
      541 gatcatcttg aagcgagcgg cggacatcgc cgaggcgctg tacagcgttt gacagtaaag
      601 gtggatacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac
      661 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      721 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      781 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      841 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      901 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
//
