LOCUS       sequence-59846-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pEGFPC4 converted Gateway Destination vector for expressing EGFP 
            tagged fusion proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZYegfp
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..157
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(688..707)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agtccggact cagatctgca gaattgatta atacaagttt gtacaaaaaa gctgaacgag
       61 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
      121 cataatactg taaaacacaa catatccagt cactatgaat caactactta gatggtatta
      181 gtgacctgta gtcgaccgac agccttccaa atgttcttcg ggtgatgctg ccaacttagt
      241 cgaccgacag ccttccaaat gttcttctca aacggaatcg tcgtatccag cctactcgct
      301 attgtcctca atgccgtatt aaatcataaa aagaaataag aaaaagaggt gcgagcctct
      361 tttttgtgtg acaaaataaa aacatctacc tattcatata cgctagtgtc atagtcctga
      421 aaatcatctg catcaagaac aatttcacaa ctcttatact tttctcttac aagtcgttcg
      481 gcttcatctg gattttcagc ctctatactt actaaacgtg ataaagtttc tgtaatttct
      541 actgtatcga cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa
      601 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
      661 cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc
      721 cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact
      781 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac
//
