LOCUS       sequence-59848-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
  TITLE     Efficient conversion of astrocytes to functional midbrain 
            dopaminergic neurons using a single polycistronic vector.
  JOURNAL   PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
            2011 Dec 9.
  PUBMED    22174877
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28719. doi"; pages: " 
            10.1371/journal.pone.0028719. Epub 2011 Dec 9"
COMMENT     Sequence Label: MAP2-CD4
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(92..133)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    159..183
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattga ggctgatcag cgggtttaaa
       61 ctcaatggtg atggcgatga tgaccggtac gcgtagaatc gagaccgagg agagggttag
      121 ggataggctt accttcgaac cgcgggccct ctagatcaac cactttgtac aagaaagctg
      181 ggttcagtgc cggcacctga cacagaagaa gatgcctagc ccaatgaaaa gcaggaggcc
      241 ggcgacgccc cccagcacaa tcagggccat tggctgcacc ggggtcgacc atgtgggcag
      301 aaccttgatg ttggattcca gcaggacctg tcccgagtca ctcagcagac actgccacat
      361 ccccgcctca gggttcagca cccacaccgc cttctcccgc ttcgagacct ttgcctcctt
      421 gttctccagt ttcaagctca gcatcagctt aggggaggtg ggtccccaca cctcacaggt
      481 caaatttttc tggagctgag tggctctcat caccaccagg ttcacttcct gatgcaactt
      541 tcctgttttc gcttcaaggg ccagggtgag gtttccagag ccagcatact gaggcaaggc
      601 ctggggcagg gtgaggtgga gcgggagctt cttgcccatc tggagcttag ggtcctgggt
      661 aacccgtttt acagacactt ccttgttctt caggtcaaag gtgatccaag acttggagga
      721 ggaagccctc tccgcctgcc accacagctc gccactgccc gtcagctttt caactgtaaa
      781 ggcgagtggg aaggagaact ccacctgttc cccctctttc ttatagacta tgctggaggc
      841 cttctggaaa gctagcacca cgatgtctat ttt
//
