LOCUS       sequence-59850-e        7013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   McKay T, Patel M, Pickles RJ, Johnson LG, Olsen JC
  TITLE     Influenza M2 envelope protein augments avian influenza hemagglutinin
            pseudotyping of lentiviral vectors.
  JOURNAL   Gene Ther. 2006 Apr;13(8):715-24.
  PUBMED    16397505
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 
            Ther."; date: "2006-04"; volume: "13(8)"; pages: "715-24"
COMMENT     Sequence Label: pEIAV-SIN6.1 CGFPW
FEATURES             Location/Qualifiers
     source          1..7013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        146..525
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        526..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1864..2167
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2168..2371
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2523..2532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2529..2531,2532..2534,2535..3248)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2529..2531,2532..2534,2535..3245)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2574..2595)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2835..2854)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3182..3203
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3450..4038
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3503..3523)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3921..3932)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      4435..4890
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4522..4541)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4732..4753
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4959..4978)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5078..5100
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5138..5156)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5224..5328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5329..5397,5398..6189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5547..5566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6360..6948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6849..6868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acagatcttc aatattggcc attagccata ttattcattg gttatatagc ataaatcaat
       61 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
      121 tcatgtccaa tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgggcactc agattctgcg gtctgagtcc cttctctgct
      781 gggctgaaaa ggcctttgta ataaatataa ttctctactc agtccctgtc tctagtttgt
      841 ctgttcgaga tcctacagtt ggcgcccgaa cagggacctg agaggggcgc agaccctacc
      901 tgttgaacct ggctgatcgt aggatccccg ggacagcaga ggagaactta cagaagtctt
      961 ctggaggtgt tcctggccag aacacaggag gacaggtaag tagggagacc ctttgacatg
     1021 gagcaaggcg ctcaagaagt tagagaaggt gacggtacaa gggtctcaga aattaactac
     1081 tggtaactgt aattgggcgc taagtctagt agacttattt catgatacca actttgtaaa
     1141 agaaaaggac tggcagctga gggatgtcat tccattgctg gaagatgtaa ctcagacgct
     1201 ggaattcggg tattgtagaa agggcaaata ggaccttgaa agagaagatt caaagtcata
     1261 gagacaacac tcaaacactg gaggcagctt tacaacttgc tctcattact tgtaacaaag
     1321 ggagggaaag tatgggagga cagacaccat gggaagtatt tatcactaat caagcacaag
     1381 taatacatga gaaactttta ctacagcaag cacaatcctc caaaaaattt tgtttttaca
     1441 aaatccctgg tgaacatgat tggaagggac ctactagggt gctgtggaag ggtgatggtg
     1501 cagtagtagt taatgatgaa ggaaagggaa taattgctgt accattaacc aggactaagt
     1561 tactaataaa gccaaattga gtattgttgc atgcatttga tatatgggat tatttggtaa
     1621 aggggtaaca tggtcagcat cgcattctat ggggggatcc cagggggaat ctcaacccct
     1681 attacccaac agtcagaaaa atctaagtgt gaggagaaca caatgtttca accttattgt
     1741 tataataatg acagtaagaa cagcatggca gaatcgaagg aagcaagaga ccaagaaatg
     1801 aacctgaaag aagaatctaa agaagaaaaa agaagaaatg actggtggaa aaaagctgca
     1861 ggtcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1921 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1981 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2041 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2101 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2161 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2221 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2281 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2341 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     2401 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac
     2461 tctagagtcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
     2521 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2581 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     2641 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2701 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     2761 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2821 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2881 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2941 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     3001 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     3061 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3121 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     3181 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     3241 acaagtaaag cggccgcgag catcttaccg ccatttattc ccatatttgt tctgtttttc
     3301 ttgatttggg tatacatttg aatgtcaata aaacaaaatg gtggggcaat catctacatt
     3361 tcatgggata tgtgattact agttcaggtg tattgccaca agacaaacat gttaagaaaa
     3421 tttcccgtta tttgcactct gttcctgtta atcaacctct ggattacaaa atttgtgaaa
     3481 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3541 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3601 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3661 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3721 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3781 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3841 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3901 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3961 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4021 tttgggccgc ctccccgcct gtttcgcctc ggcgtccggt ccgtgttgct tggtcttcac
     4081 ctgtgcagac ttgcgaacca tggattccac cgtgaacttt gtctcctggc atgcaaatcg
     4141 tcaacttggc atgccaaatc gattagaaaa acaagggggg aactgtgggg tttttatgag
     4201 gggttttata caattgggca ctcagattct gcggtctgag tcccttctct gctgggctga
     4261 aaaggccttt gtaataaata taattctcta ctcagtccct gtctctagtt tgtctgttcg
     4321 agatcctaca gagctcatgc cttggttaat taaggagatc cgggctggcg taatagcgaa
     4381 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
     4441 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4501 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4561 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagagctt
     4621 tacggcacct cgaccgcaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     4681 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4741 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4801 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaata tttaacgcga
     4861 attttaacaa aatattaacg tttacaattt cgcctgatgc ggtattttct ccttacgcat
     4921 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     4981 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     5041 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     5101 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     5161 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5221 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5281 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5341 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5401 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5461 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5521 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5581 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5641 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5701 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5761 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5821 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5881 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5941 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6001 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6061 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6121 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6181 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6241 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6301 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6361 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6421 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6481 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     6541 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6601 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6661 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6721 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6781 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6841 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6901 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6961 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc tcg
//
