LOCUS       sequence-59851-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
  TITLE     Efficient conversion of astrocytes to functional midbrain 
            dopaminergic neurons using a single polycistronic vector.
  JOURNAL   PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
            2011 Dec 9.
  PUBMED    22174877
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28719. doi"; pages: " 
            10.1371/journal.pone.0028719. Epub 2011 Dec 9"
COMMENT     Sequence Label: tetO-ALN
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..91)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(101..142)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgag gctgatcanc
       61 gggtttaaac tcaatggtga tggtgatgat gaccggtacg cgtagaatcg agaccgagga
      121 gagggttagg gataggctta ccttcgaacc gcgggccctc tagatcaacc actttgtaca
      181 agaaagctgg gtagaaaggt aaagtgtcca ggaaaagttt gtcaattatt gctggcggtg
      241 gcaccaagtc ttccaatttc aggtagaaaa tgcgctgtag cccctgtgtg caaagggtac
      301 gaagttctgg gagcttcccc aacagtttgg acaaataatt ggggcggttc aaccccccat
      361 tgttgaaagt cacgtggtct ttgagacaat ttacaatctt gttttgcagt tcttccactc
      421 tcttgggttc cttgagcccg tgtctctctg tgaccatagc cagggcagca atgcaggaga
      481 aggcagaaat gtcgatgttc atattctgca agttggagga gaattcaaca atggaatcaa
      541 tccattcccc aaagccacga acgcattgca acctgtgcaa gaccacccca ttgcaaaaga
      601 tgagtttacc ctccactggg ttggacctgt atgctaatcg aaggacaaac agttctaaga
      661 aagctgattc aaaaagcagg tcttggtcgg ctttgggcag gtctgcgaag ccagggatct
      721 tctctgccca gccccggatg atctccatgg agccagtcag gagatcatag aattgctgga
      781 tatgctgggt gtcatctcca ctcatttgat agtcagggtt cgcctggaac ctggaatagt
      841 ccaggctggt catagccggg
//
