LOCUS       sequence-59859-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
  TITLE     Efficient conversion of astrocytes to functional midbrain 
            dopaminergic neurons using a single polycistronic vector.
  JOURNAL   PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
            2011 Dec 9.
  PUBMED    22174877
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28719. doi"; pages: " 
            10.1371/journal.pone.0028719. Epub 2011 Dec 9"
COMMENT     Sequence Label: MAP2-GCaMP3
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..39
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..75
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(79..87,88..102)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             130..186
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     399..420
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(526..547)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(787..806)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaaaagcagg ctatgggttc tcatcatcat catcatcatg gtatggctag catgactggt
       61 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatctcgc caccatggtc
      121 gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat aggtcggctg
      181 agctcactcg agaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
      241 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
      301 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag
      361 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      421 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
      481 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      541 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
      601 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      661 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      721 cagcacgact tcttcaagtc cgccatgccc gtaggctaca tccaggagcg caccatcttc
      781 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      841 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctgggg
//
