LOCUS       sequence-59871-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
  TITLE     Efficient conversion of astrocytes to functional midbrain 
            dopaminergic neurons using a single polycistronic vector.
  JOURNAL   PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
            2011 Dec 9.
  PUBMED    22174877
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28719. doi"; pages: " 
            10.1371/journal.pone.0028719. Epub 2011 Dec 9"
COMMENT     Sequence Label: tetO-ALN
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(214..270)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gcagctggtg gtgagggctt gctgacacgc cgcgcactgc aaacactcct cgtgccagga
       61 cgactcgttg actcgcatca ggaagcggtc ggagatgggc cgctggcagc cctcgcagac
      121 ggcgggatgc gggcagtcgg agcccagcag cacccccaga gtggcgggcc cggggcgcag
      181 ggcgtgctcc tccatcttga tgccgtccaa cataggcccg gggttttctt caacatctcc
      241 tgcttgcttt aacagagaga agttcgtggc accggatccg aaccagttgg tgaagtcgag
      301 aagctcctgc tcctcggggc tgagcgggtc gtaagagccc tcgtccgacg agtaggatga
      361 gaccggcgag ccggccatgg agttcaagtc gttggagtag ttgggggaga tggtgggcga
      421 caggacgcct gcctggaagg cggcgctcac cgcgtcatgc tcgtccagca gctgctgcag
      481 cgcgcggatg tactcgaccg ccgagcgcag tgtctccacc ttactcatct tcttgttggc
      541 cgcgccgttg gggacgtgct cccgaagggt ggcaaagccc aggttgacca acttgacgcg
      601 gttgcgctcg cgctcgttgc ggcgcgccac ggcggccggc tgctgctgcg gcaggctgta
      661 gccaaagccg ctgaagttga gccggcgttt gcagcgcatc agttcgggcg aagacgagcg
      721 ctgtcgcttg acttgcttgg gcgctgactt gtgaccgccc cctgagggct ggccgtcggc
      781 cgccggtctc agctgcggcg cctgctgctg ctgctgctgc tgctgctgct gctgctgcgc
      841 gctctgcgct gccgctgcgg cggctgcggc cgccgcggct gcggccgtgg caaagaaaca
      901 ggctgcgggc ggca
//
