LOCUS       sequence-59892-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AHCYL1A
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..103
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             110..130
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtt ggtcccatcc gcaatttgaa aagggtaccg agaacctgta
      121 cttccaatcc atggtttctc cccgagagaa gcagcaaacc aactccaagg gcagcagcaa
      181 tttctgtgtg aagaacatca agcaggcaga atttggacgc cgggagattg agattgcaga
      241 gcaagacatg tctgctctga tttcactcag gaaacgtgct cagggggaga agcccttggc
      301 tggtgctaaa atagtgggct gtacacacat cacagcccag acagcggtgt tgattgagac
      361 actctgtgcc ctgggggctc agtgccgctg gtctgcttgt aacatctact caactcagaa
      421 tgaagtagct gcagcactgg ctgaggctgg agttgcagtg ttcgcttgga agggcgagtc
      481 agaagatgac ttctggtggt gtattgaccg ctgtgtgaac atggatgggt ggcaggccaa
      541 catgatcctg gatgatgggg gagacttaac ccactgggtt tataagaagt atccaaacgt
      601 gtttaagaag atccgaggca ttgtggaaga gagcgtgact ggtgttcaca ggctgtatca
      661 gctctccaaa gctgggaagc tctgtgttcc ggccatgaac gtcaatgatt ctgttaccaa
      721 acagaagttt gataacttgt actgctgccg agaatccatt ttggatggcc tgaagaggac
      781 cacagatgtg atgtttggtg ggaaacaagt ggtggtgtgt ggctatggtg aggtaggcaa
      841 gggctgctgt gctgctctca aagctcttgg agcaattgtc tacattaccg aaatcgaccc
      901 catctgtgct ctgcaggcct g
//
