LOCUS       sequence-59963-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBF1A
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             466..483
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(536..554)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      550..597
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(721..740)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     931..952
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgga
      121 acatgctact ccctgtatca aagccatcag cccgagtgaa ggatggacga cgggaggtgc
      181 gactgtgatc atcatagggg acaatttctt tgatgggtta caggtcatat tcggtaccat
      241 gctggtctgg agtgagttga tcactcctca tgccatccgt gtgcagaccc ctcctcggca
      301 catccctggt gttgtggaag tcacactgtc ctacaaatct aagcagttct gcaaaggaac
      361 accaggcaga ttcatttata cagcgctcaa cgaacccacc tgacagtaaa ggtggatacg
      421 gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac
      481 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      541 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      601 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      661 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      721 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      781 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      841 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      901 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaa
//
