LOCUS       sequence-60008-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   von Gise A, Lin Z, Schlegelmilch K, Honor LB, Pan GM, Buck JN, Ma Q,
            Ishiwata T, Zhou B, Camargo FD, Pu WT
  TITLE     YAP1, the nuclear target of Hippo signaling, stimulates heart growth
            through cardiomyocyte proliferation but not hypertrophy.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2394-9. doi: 
            10.1073/pnas.1116136109. Epub 2012 Jan 30.
  PUBMED    22308401
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1116136109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-02-14- 14"; volume: "109"; issue: "7"; pages: "2394-9"
COMMENT     Sequence Label: pENTR-3xFLAG-YAP1-S127A
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             126..197
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctctttaa aggaaccaat tcagtcgaca
      121 ccatggacta caaggacgac gatgacaagg attacaaaga tgacgacgat aaggactata
      181 aggacgatga tgataaactg gtacctatgg atcccgggca gcagccgccg cctcaaccgg
      241 ccccccaggg ccaagggcag ccgccttcgc agcccccgca ggggcagggc ccgccgtccg
      301 gacccgggca accggcaccc gcggcgaccc aggcggcgcc gcaggcaccc cccgccgggc
      361 atcagatcgt gcacgtccgc ggggactcgg agaccgacct ggaggcgctc ttcaacgccg
      421 tcatgaaccc caagacggcc aacgtgcccc agaccgtgcc catgaggctc cggaagctgc
      481 ccgactcctt cttcaagccg ccggagccca aatcccactc ccgacaggcc agtactgatg
      541 caggcactgc aggagccctg actccacagc atgttcgagc tcatgcctct ccagcttctc
      601 tgcagttggg agctgtttct cctgggacac tgacccccac tggagtagtc tctg
//
