LOCUS       sequence-60018-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Beier KT, Saunders A, Oldenburg IA, Miyamichi K, Akhtar N, Luo L, 
            Whelan SP, Sabatini B, Cepko CL
  TITLE     Anterograde or retrograde transsynaptic labeling of CNS neurons with
            vesicular stomatitis virus vectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9. Epub 2011 Aug
            8.
  PUBMED    21825165
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-09-13"; volume: "108(37)"; pages: 
            "15414-9. Epub 2011 Aug 8"
COMMENT     Sequence Label: pVSV Venus VSV-G
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2..4,5..7,8..718)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(47..68)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(308..327)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     655..676
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
       61 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      121 cggcaagctg accctgaagt taatctgcac caccggcaag ctgcccgtgc cctggcccac
      181 cctcgtgacc accttaggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
      241 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      301 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      361 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      421 caagctggag tacaactaca acagccacaa cgtctatatc acggccgaca agcagaagaa
      481 cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
      541 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      601 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      661 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
//
