LOCUS       sequence-60043-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B
  TITLE     An improved short-lived fluorescent protein transcriptional reporter
            for Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 
            21.
  PUBMED    23172645
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2932"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "519-30"
COMMENT     Sequence Label: pNC1011
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..739
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     679..699
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcgacggatc cccgggttaa ttaacatgag taaaggagaa gaacttttca ctggagttgt
       61 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      121 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
      181 actacctgtt ccatggccaa cacttgtcac tactctcact tatggtgttc aatgcttttc
      241 aagataccca gatcacatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
      301 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
      361 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
      421 agatggaaac attcttgggc acaaattgga atacaactat aactcacaca atgtatacat
      481 catggcagac aaacaaaaga atggaatcaa agctaacttc aaaattagac acaacattga
      541 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      601 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
      661 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
      721 catggatgaa ctatacaaaa gatccatgag taaaggagaa gaacttttca ctggagttgt
      781 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      841 gggtgaaggt gatgcaacat acgga
//
