LOCUS       sequence-60090-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUDT10A
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(106..123)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             553..575
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    590..614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(614..633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(615..633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(717..736)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcagcttcct ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg
       61 agtgcggccg caagcttgtc gacggagctc gaattcggat cctcaatggt ggtgatgatg
      121 gtgcgccttt agtttctcca gatattcggc gtgcacgggc ttgtggcact ggagaacctt
      181 gatggcatct tcgactttga accactctcg cttcctccca atgctaaccg aatcttccca
      241 atcctccagc agctccgtga cagtcagtac atacacgtac gttctgtgct cggggtcctg
      301 gttctgttcg aagacgccca ggagccggcc taacttcccc ttgactcccg cctcttcgta
      361 cacctctcgg accgccgcac cgcccggctc ctcctcgggc tccatgcccc cgcccggcac
      421 gatccagcgg tccgggtacc ggctgctact cactaacagg acctcgtcct cgcgctcgct
      481 ccggaagcac aggcacgccg cccgcttctt cattaattat ctcctctttg tgattcatga
      541 ttcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      601 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      661 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      721 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      781 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
      841 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
      901 cctaatgcag gagtcgcata agggagagcg tcgagatc
//
