LOCUS       sequence-60091-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NVLA
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgac
      121 tgtccctaat gtgacatggg cagatattgg tgccctggaa gacattagag aggagctcac
      181 catggcaata ttggcaccag tacgcaaccc agaccagttc aaagctcttg gattggtgac
      241 tccagctggg gtcctccttg ctggtcctcc tggctgtggg aagactctgc tggcgaaggc
      301 tgttgcaaat gagtccggac taaattttat atctgtcaag ggccccgaat tactaaacat
      361 gtatgttggt gagagtgaac gtgctgtgcg acaagttttt caacgagcca agaactcagc
      421 accctgtgtg atattctttg atgaagtgga tgctttatgt cctcgaagat cagaccgaga
      481 gacaggggca agtgtccgag tggtgaatca gctacttaca gagatggatg gtctggaagc
      541 acgccagcag gtttttatta tggcagccac taacaggcca gatataattg accctgcaat
      601 cctgcgcccg ggccgcctgg acaaaacact gtttgtgggt ttaccgcccc ctgcagatcg
      661 ccttgccatc ttaaaaacta tcacaaaaaa tggtaccaaa ccaccactgg atgcagatgt
      721 aaatttggaa gcaattgctg gtgaccttcg ctgtgattgc tatacgggcg cagatctctc
      781 tgctttggta cgagaagctt ctatctgtgc cctgagacag gaaatggcaa gacagaagag
      841 tggaaatgaa aaaggtgaac tcaaggttag tcataagcat tttgaagaag ctttc
//
