LOCUS       sequence-60092-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBF1A
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             907..924
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgcg
      121 gagtggaagc agcatgaagg aagagccgct gggcagcggc atgaacgcgg tgcggacgtg
      181 gatgcagggc gccggggtgc tggacgccaa cacggcggcg cagagcgggg tgggtctggc
      241 ccgggctcac tttgagaagc agccgccttc caatctgcgg aaatccaact tcttccactt
      301 cgtcctggcc ctctacgaca gacagggcca gcccgtggag atcgagagga cagcgtttgt
      361 ggggttcgtg gagaaggaaa aagaagccaa cagcgaaaag accaataacg gaattcacta
      421 ccggcttcag cttctctaca gcaatgggat aaggacggag caggatttct acgtgcgcct
      481 cattgactcc atgacaaaac aagccatagt gtatgaaggc caagacaaga acccagaaat
      541 gtgccgagtc ttgctcacac atgagatcat gtgcagccgc tgttgtgaca agaaaagctg
      601 tggcaaccga aatgagactc cctcagatcc agtgataatt gacaggttct tcttgaaatt
      661 tttcctcaaa tgtaaccaaa attgcctaaa gaatgcggga aacccacgtg acatgcggag
      721 attccaggtc gtggtgtcta cgacagtcaa tgtggatggc catgtcctgg cagtctctga
      781 taacatgttt gtccataata attccaagca tgggcggagg gctcggaggc ttgacccctc
      841 gtgacagtaa aggtggatac ggatccgaat tcgagctccg tcgacaagct tgcggccgca
      901 ctcgagcacc accaccacca ccactgagat ccg
//
