LOCUS       sequence-60094-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIK3C3A
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgag
      121 caaacaccac aagcttgccc ggagtttaag aagtggacct tctgaccacg atctgaaacc
      181 caatgctgcc acgagagatc agttaaatat tattgtgagt tatccaccaa ccaagcaact
      241 tacatatgaa gaacaagatc ttgtttggaa gtttagatat tatcttacga atcaagaaaa
      301 agccttgaca aaattcttga aatgtgttaa ttgggatcta cctcaagagg ccaaacaggc
      361 cttggaactt ctgggaaaat ggaagccgat ggatgtagag gactccttgg agctgttatc
      421 ctctcattac accaacccaa ctgtgaggcg ttatgctgtt gcccggttgc gacaggccga
      481 tgatgaggat ttgttgatgt acctattaca attggtccag gctctcaaat atgaaaattt
      541 tgatgatata aagaatggat tggaacctac caagaaggat agtcagagtt cagtgtcaga
      601 aaatgtgtca aattctggaa taaattctgc agaaatagat agctcccaaa ttataaccag
      661 cccccttcct tcagtctctt cacctcctcc tgcatcaaaa acaaaagaag ttccagatgg
      721 cgaaaatctg gaacaagatc tctgtacctt cttgatatcg agagcctgca aaaactcaac
      781 actggctaat tatttatact ggtatgtgat agtggaatgt gaagatcaag atactcagca
      841 gagagatcca aagacccatg agatgtactt gaacgtaatg agaagattca gccaagcatt
      901 gttgaagggt gataagtctg tcagagttat gcg
//
