LOCUS       sequence-60100-e        3716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Mergenthaler P, Muselmann C, Sunwoldt J, Isaev NK, Wieloch T, 
            Dirnagl U, Meisel A, Ruscher K
  TITLE     A functional role of the cyclin-dependent kinase inhibitor 1 
            (p21(WAF1/CIP1)) for neuronal preconditioning.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Mar;33(3):351-5. doi: 
            10.1038/jcbfm.2012.213. Epub 2013 Jan 9.
  PUBMED    23299246
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2012.213"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-03"; volume: "33"; issue: "3"; pages: "351-5"
COMMENT     Sequence Label: pCAG-p21_T145A/S146A
FEATURES             Location/Qualifiers
     source          1..3716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        314..591
                     /label=chicken beta-actin promoter
     primer_bind     1534..1556
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1664..2158
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQAAMTDFYHSKRRLIFSKRK
                     P"
     primer_bind     complement(2208..2227)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2541..3129
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3030..3049
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3215..3262
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3254..3272
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
       61 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      121 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      181 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      241 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      301 gctattaaca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
      361 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
      421 gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg
      481 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
      541 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc ggggagtcgc
      601 tgcgacgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
      661 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
      721 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
      781 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
      841 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
      901 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
      961 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1021 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     1081 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     1141 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     1201 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     1261 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1321 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     1381 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1441 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     1501 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1561 gctctagaca attgtactaa ccttcttctc tttcctctcc tgacaggttg gtgtacagta
     1621 gcttccacca tggactcaga tctcgagctc aagcttcgaa ttcatgtcag aaccggctgg
     1681 ggatgtccgt cagaacccat gcggcagcaa ggcctgccgc cgcctcttcg gcccagtgga
     1741 cagcgagcag ctgagccgcg actgtgatgc gctaatggcg ggctgcatcc aggaggcccg
     1801 tgagcgatgg aacttcgact ttgtcaccga gacaccactg gagggtgact tcgcctggga
     1861 gcgtgtgcgg ggccttggcc tgcccaagct ctaccttccc acggggcccc ggcgaggccg
     1921 ggatgagttg ggaggaggca ggcggcctgg cacctcacct gctctgctgc aggggacagc
     1981 agaggaagac catgtggacc tgtcactgtc ttgtaccctt gtgcctcgct caggggagca
     2041 ggctgaaggg tccccaggtg gacctggaga ctctcagggt cgaaaacggc ggcaggccgc
     2101 catgacagat ttctaccact ccaaacgccg gctgatcttc tccaagagga agccctaagg
     2161 atccaccggt cgccagcggc cgctagctcg acatgataag atacattgat gagtttggac
     2221 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     2281 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2341 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2401 ggttttttaa agcaagtaaa acctctacaa atgtggtaga tccatttaaa tgttaattaa
     2461 ctagccatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2521 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2581 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2641 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2701 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2761 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2821 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2881 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2941 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3001 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3061 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3121 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3181 gctcacatgt tcttaattaa atttttcaaa agtagttgac aattaatcat cggcatagta
     3241 tatcggcata gtataatacg actcactata ggagggccat catggccaag ttgaccagtg
     3301 ctgtcccagt gctcacagcc agggatgtgg ctggagctgt tgagttctgg actgacaggt
     3361 tggggttctc cagagatttt gtggaggatg actttgcagg tgtggtcaga gatgatgtca
     3421 ccctgttcat ctcagcagtc caggaccagg tggtgcctga caacaccctg gcttgggtgt
     3481 gggtgagagg actggatgag ctgtatgctg agtggagtga ggtggtctcc accaacttca
     3541 gggatgccag tggccctgcc atgacagaga ttggagagca gccctggggg agagagtttg
     3601 ccctgagaga cccagcaggc aactgtgtgc actttgtggc agaggagcag gactgaggat
     3661 aagaattgag tttcagaaaa gggggcctga gtggcccctt ttttcaactt aattaa
//
