LOCUS       sequence-60102-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Moleirinho S, Chang N, Sims AH, Tilston-Lunel AM, Angus L, Steele A,
            Boswell V, Barnett SC, Ormandy C, Faratian D, Gunn-Moore FJ, 
            Reynolds PA
  TITLE     KIBRA exhibits MST-independent functional regulation of the Hippo 
            signaling pathway in mammals.
  JOURNAL   Oncogene. 2012 May 21. doi: 10.1038/onc.2012.196.
  PUBMED    22614006
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2012 May 21. doi: 10.1038/onc.2012.196."
COMMENT     Sequence Label: pLKO.1puro-shKIBRA-A
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    132..249
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        298..808
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     727..745
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 tggctttata tatcttgtgg aaaggacgaa acaccgggct tcactgacct ctactattca
       61 agagatagta gaggtcagtg aagctttttt gaattctcga cctcgagaca aatggcagta
      121 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      181 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      241 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg gctcgagggg
      301 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
      361 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
      421 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
      481 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
      541 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
      601 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
      661 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
      721 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
      781 tgaccgaatc accgacctct ctccccaggg ggatccaccg gagcttacc
//
