LOCUS       sequence-60115-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Anastasiou D, Poulogiannis G, Asara JM, Boxer MB, Jiang JK, Shen M, 
            Bellinger G, Sasaki AT, Locasale JW, Auld DS, Thomas CJ, Vander 
            Heiden MG, Cantley LC
  TITLE     Inhibition of pyruvate kinase M2 by reactive oxygen species 
            contributes to cellular antioxidant responses.
  JOURNAL   Science. 2011 Dec 2;334(6060):1278-83. doi: 10.1126/science.1211485.
            Epub 2011 Nov 3.
  PUBMED    22052977
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1211485"; journalName: "Science"; date: "2011-12-2-
            2"; volume: "334"; issue: "6060"; pages: "1278-83"
COMMENT     Sequence Label: pLHCX-Flag-mPKM2(K433E)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             75..98
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cccaagcttg
       61 ttaacgccac catggactac aaagacgatg acgataaagc aagggatcca ctagtaacgg
      121 ccgccagtgt gctggaattc atgccgaagc cacacagtga agcagggact gccttcattc
      181 agacccagca gctccatgca gccatggctg acaccttcct ggaacacatg tgccgcctgg
      241 acattgactc tgcccccatc acggcccgca acactggcat catttgtacc attgggcctg
      301 cttcccgatc tgtggagatg ctgaaggaga tgattaagtc tggaatgaat gtggctcggc
      361 tgaatttctc tcatggaacc catgagtacc atgcagagac catcaagaat gtccgtgaag
      421 ccacagaaag ctttgcatct gatcccattc tctaccgtcc tgttgcggtg gctctggata
      481 caaagggacc tgagatccgg actggactca tcaagggcag cggcaccgct gaggtggagc
      541 tgaagaaggg agccactctg aagatcaccc tggacaacgc ttacatggag aagtgtgacg
      601 agaacatcct gtggctggac tacaagaaca tctgcaaggt ggtggaggtg ggcagcaaga
      661 tctacgtgga cgatgggctc atctcactgc aggtgaagga gaaaggcgct gacttcctgg
      721 tgacggaggt ggagaatggt ggctccttgg gcagcaagaa gggcgtgaac ctgccgggcg
      781 ctgctgtgga tctccccgct gtgtcggaaa aggacatcca ggacctgaag tttggggtgg
      841 agcaggatgt gg
//
