LOCUS       sequence-60122-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Anastasiou D, Poulogiannis G, Asara JM, Boxer MB, Jiang JK, Shen M, 
            Bellinger G, Sasaki AT, Locasale JW, Auld DS, Thomas CJ, Vander 
            Heiden MG, Cantley LC
  TITLE     Inhibition of pyruvate kinase M2 by reactive oxygen species 
            contributes to cellular antioxidant responses.
  JOURNAL   Science. 2011 Dec 2;334(6060):1278-83. doi: 10.1126/science.1211485.
            Epub 2011 Nov 3.
  PUBMED    22052977
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1211485"; journalName: "Science"; date: "2011-12-2-
            2"; volume: "334"; issue: "6060"; pages: "1278-83"
COMMENT     Sequence Label: pET28a-His-hPKM2
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgtc taaaccgcac tctgaagctg
      121 gtaccgcctt cattcagacc cagcaactgc acgcagccat ggctgacaca ttcctggagc
      181 acatgtgccg cctggacatt gattcaccac ccatcacagc ccggaacact ggcatcatct
      241 gtaccattgg cccagcttcc cgatcagtgg agacgttgaa ggagatgatt aagtctggaa
      301 tgaatgtggc tcgtctgaac ttctctcatg gaactcatga gtaccatgcg gagaccatca
      361 agaatgtgcg cacagccacg gaaagctttg cttctgaccc catcctctac cggcccgttg
      421 ctgtggctct agacactaaa ggacctgaga tccgaactgg gctcatcaag ggcagcggca
      481 ctgcagaggt ggagctgaag aagggagcca ctctcaaaat cacgctggat aacgcctaca
      541 tggaaaagtg tgacgagaac atcctgtggc tggactacaa gaacatctgc aaggtggtgg
      601 aagtgggcag caagatctac gtggatgatg ggcttatttc tctccaggtg aagcagaaag
      661 gtgccgactt cctggtgacg gaggtggaaa atggtggctc cttgggcagc aagaagggtg
      721 tgaaccttcc tggggctgct gtggacttgc ctgctgtgtc ggagaaggac atccaggatc
      781 tgaagtttgg ggtcgagcag gatgttgata tggtgtttgc gtcattcatc cgcaaggcat
      841 ctgatgtcca tgaagttagg aaggtc
//
